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Presentation on In Vitro Molecular Biology 3/5/03

Creating "Life" in a Test Tube

Kimberley, Michael, LeRoy, Naomi, Greg


Using available sequence data a polio virus was created from scratch. What does this mean in terms of other viruses or organisms being created in the same way? Craig Venter has also proposed using a similar idea to create new bacteria which could be used to generate hydrogen fuel.


Articles of interest:

Dangerous Virus made from mail-order kits. Should this have been done? Cell News, October, 2002. http://www.geocities.com/giantfideli/cellnews_dangerous_virus_made_from_mail-order_kits.html

Genome man to create new life. BBC News, November, 2002. http://news.bbc.co.uk/2/low/science/nature/2499119.stm

 

1. Key issues and ethical problems:

  • Creating life can be done by the piecing together of the correct genetic sequences in an appropriate environment. This is difficult and seems to only be a viable method for creating organisms with very small genomes i.e.: viruses or bacteria.
  • There are ethical problems such as if the information of sequences is available can our enemies use such technology to create viruses to use for bioterrorism? Should then this sequence data be restricted in terms of public access? If so, who would monitor what is safe, and how would research be hindered? Furthermore, if it can be, done does information restriction really help us at this point or do we need to keep things available to help ensure we can find ways to respond to bioterrorist threats?
  • There is also the ethical issue for many that man has no right to be creating "new" life, that it is God's work.
  • Lastly, we need to consider what benefits may be gained by this technological capability.

2. Background inforomation:

  • How difficult is this process molecularly and just what kind of viruses or "life" could be created? Only things with small genomes such as bacteria and viruses, although perhaps bacteria would largely just be a considerable genetic modification rather than created completely de novo as the polio virus was.
  • Where should the line be drawn between the right to information exchange and the need to protect the public from such information? All sequence inforomation isn't "evil" so where can the line be drawn?
  • What benefits are potentially gained? If in fact we could create bacteria that pose no risk to humans or the environment, but they could help to generate fuel or be used for cleaning up the environment is it really bad? There are lots of ways in which bacteria are utilized for industrial and bioremediation purposes and they have worked quite well. We already try to use viruses without the virulence genes to create vaccines, so is being able to create one de novo all that different from where we have been in terms of being able to modify what's already there?
  • Is there any way to really prevent viruses from being created? For example, even if we went to the extreme and abolished all sequence information on the web for public access it doesn't mean that the information cannot be gathered from other resources or privately funded research. Limiting sequence data for extremely dangerous pathogens such as small pox is comforting, but people can do their own sequencing as well.
  • We can make stock houses report what they sell if the sequence may be suspicious, or not sell particular oligos with a potential connection to very dangerous pathogens, but not only is this a slippery slope, but we cannot police other countries and we might not be able to enforce local restrictions either.

3. Experts:

a. Someone who can represent the benefits of such work.

b. Someone who can represent the risks of such work, especially in terms of what kinds of bioweapons or health threats could be created.

c. Someone to look at the laws and see if we need to place stricter regulations or it its even possible with the current infrastructure and budget.

4. Values:

  • Is it okay to create life? We have already been engineering vectors and such for some time, so what is the distinction here?
  • Is the value of scientific advancement high enough to accept the inherent risks that may accompany it?
  • Is there a tendency for the public to overreact because they don't understand all the details? Opinions on some post-your-thoughts sites seem to regard sequence data on the web as being analogous to posting instructions on how to build a pipe bomb.

5. Possible Solutions:

  • Try to find a way to set up watch dogs with regard to data or supplies for potentially dangerous purposes. This means making sure that something virulent like the data for small pox remains protected.
  • Make sure that if bacteria are created for technological solutions that they are in fact incapable of surviving outside of their narrowly controlled environment, they do not have any virulence, and that they will indeed serve a legitimate purpose before being made.
  • Try to educate the public about anything made, and all aspects of it, to prevent panic where it is not necessary and address concerns when necessary.

 

6. Organization of decision making process:

  • Government agencies and organizations such as WHO, CDC, USAMRIID need to be capable of safeguarding info and deciding what access to it is permissible.
  • Perhaps a panel of appropriately knowledgeable individuals can decide on policy for sales of oligos and tools to help safeguard, if this is really even feasible.
  • As a society we need to know what the dangers are and have people working to find solutions to bioterrorism before it happens. We've probably already past the point of no return and we should realize this and try to seek solutions.

 

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