From the outside looking in: Hershey-Chase experiment with T2 phage Replication cycle: see Table 6.1 for a list of some vertebrate
receptors attachment proteins of virus- part of capsid,
envelope protein, peplomer Penetration: [plants need direct breach of
cell membrane] 2. translocation of entire virus via cell
receptor 3. endocytosis of virus via coated pits- most
common 4. fusion of viral envelope c membrane uncoating- release of genome into cytoplasm
[usually] Replication/gene expression: eclipse phase of
growth curve Assembly: combining of capsid proteins,
joining/entry of genome maturation- final processing for virion to become
infectious Release: a.k.a. burst budding- shedding may occur over long period exocytosis- release via vesicle fusion c cell
membrane cell-cell transmission- cell/cell contact Bring CRC book to class Tuesday to play "Name That
Virus"
Defining replication vs. infection:replication- making copies of new virion
infection- host cell vs. host organismgrowth curves- timing & numbers
shift in cellular processes during virus infection
Attachment: required for all except plant
viruses [why?]
sites; cell receptors
1. injection of genome while capsid remains
outside
a few release into nucleus; a few don't
uncoat
replication strategy is part of
classification scheme
assembly sites recognized in cells as
inclusion bodies
lysis c rapid release of progeny [hence
"burst"]
Download ICTV Virus Key: http://www.ncbi.nlm.nih.gov/ICTV/new_virkey.html
Next week:Taxonomy/systematics- Wagner, ch 5; NCBI &
ICTV web sites; CRC, ch 2, table 1
Non-enveloped [+] strand RNA viruses- Wagner, ch
15; CRC, ch 7
Updated 1/25/04 by thatcher@sonoma.edu