Structure: identification of parts & basics of assembly examples of representative viruses Genomic structure & organization covered next week
[Browse Wagner, Part 4] Assignments on structure: 2. Build a 3D model of a virus of your choosing to
show next week Intro to basic methods & terminology Terminology 101: in vitro: no live cells Mass & dimensions: kilobases or kilobase pairs: nucleic acids Viral component purification: valuable info on structure
& components Quantitative methods: # particles vs. infective units Do 1st: What are some of the problems to isolating &
ID of virus[es]? How do you quantify viruses? Do next: Prepare for discussion next time Why did you choose these methods? In what sequence would you use the selected
methods? How long will it take to see results of the
assays? Resources: Material & experiences from other courses
[biol/chem]
INTRODUCTION OF METHODS
Overview of variety of virus shapes & sizes2 basic forms- helix & isometric
[cubic] (rod vs. spherical)
binary symmetry- combination of helix &
isometric
1. Computer Exercise 1 involves computer models
& EM/SEM of viruses
in vivo: living cells- natural or cultured
freq. refs to molecular weights or mass
(daltons): proteins
modes of infection differ depending on host type
dose response curves vs. systemic infections
In-class discussion this week: How do you isolate
& characterize the viruses?Object- Identify methods & reason for
choices
Why do you need to purify the virus?
Identify methods appropriate for your problem
Wagner, Part 3
Levy, Appendix 1- on reserve
Web links &/or do a general search
Updated 1/25/04 by thatcher@sonoma.edu