SSU home

..Biology Department home

 

Virology

Home | Index | Syllabus | Schedule | Study aids | Computing | Links | Interactive

 

Spring, 2004 Lecture Outline

3/30 PARVOVIRIDAE & PAPOVAVIRIDAE


Parvoviridae
: literally means "small virus"

3 genera:
parvoviruses- mammals
densoviruses- arthropods
adeno-assoc viruses (AAV)- mammals & man
dependent on adeno- or herpesviruses

generally non-path to adults; young at risk

feline parvo- panleukopenia: all types of WBC low
dogs- thought to be mutant of mink parvo: worldwide since 1978

human- B19 1st noted in 1975 in blood donors [sans any Sx]

B19 causes acute aplastic phase in several hereditary
hemolytic Dz's; fifth disease

structure: ss DNA 1.5 x 106 d in interesting shape- not circular,

organized in Y-shaped hairpin folds

usually minus strand- mRNA copies made directly
capsid- icosahedral assembly of 2-3 large proteins
Y-shaped DNA: appears to aid in integration & latency

3 open reading frames; overlapping & nested mRNAs

replication: occurs in nucleus, host enzymes

usually only during S & G2 phase of cell cycle

transcription - assembly:

transcription only from ds forms of DNA [RF]

assembly is in nucleus


Dz: aplastic crisis- in pts c variety of hemolytic Dz's:

sickle cell most common assoc.
fifth Dz: #5 rash p rubella, morbilli measles, scarlet fever, pseudoscarlatina

both transmitted easily within families; course lasts 3 wks
both produce lasting immunity


Papovaviridae: Big news!! dsDNA!! circular: models for cellular DNA

can transform cells in some cases

papilloma grp- warts, cervical CA, papillomas, skin tumors

larger in size; genomic info all on 1 strand
rare if at all integrated in host DNA; remains as plasmid

polyoma grp- multifocal tumors, lymphomas

genomic info on 2 strands (compare c ambisense)

Papilloma viruses:

large number of species & apparent strains if in preferred host- little damage
if in different host- freq. malignancies

target cell- differentiating epithelial cells in basal layer

structure & replication:

not studied as much as polyomas due to restricted cell tropism

icosahedral capsid c 1 major protein monomer

Polyoma viruses:

some are easily isolated from animals showing no Sx
some produce tumors in variety of organs

transformation c no replication

** in contrast to RNA viruses which transform & replicate

prototype: SV40- simian virus

structure & replication: [Note: other polyoma & SV40- not close relatives]

icosahedral capsid c 3 proteins
circular ds DNA can integrate into host genome
genes for structural proteins: show overlap & read-thru

replication by theta mode

do not req. hosts actively synth DNA as do parvo-

transcription & translation:

polyoma are smallest which regulate for early & late RNAs
1. early- close to origin, counterclockwise
2. late- opposite direction, clockwise on other strand
3. untranscribed- mid section: many housekeeping fxns

early genes --> 2 proteins: T & t [tumor Ags]
late genes --> 3 coat proteins

abortive infection & transformation: E.g.: JC virus trophic for glials

 
Next up: Adeno- & Herpes- viridae; Baculo- & Irido- viridae

 

Home | Index | Syllabus | Schedule | Study aids | Computing | Links | Interactive

 Updated 1/25/04 by thatcher@sonoma.edu