in montane leaf beetles
Sonoma State University
Study Objectives (Page 4 of 4)
We will investigate thermal adaptation in
natural populations ofChrysomela
aeneicollis, in the eastern
Sierra Nevada, California. To accomplish this goal, we will
investigate quantitative physiological characters that
change in response to environmental temperature and
biochemical characters that vary genetically among natural
populations of beetles. Our specific objectives are
- Determine how genetic variation in
the polymorphic enzyme phosphoglucose isomerase (PGI) relates to thermal stress
- Characterize effects of temperature
variation on heat shock protein expression, PGI activity,
and kinetics of the main PGI alloforms.
- Quantify local adaptation in thermal
tolerance by transplanting genotypes among heterogeneous
environments and measuring their survival and
- Expand population surveys of allele
frequency and environmental temperature into new
drainages to determine the degree of local thermal
adaptation to those environments.
To accomplish these objectives, we will
conduct field and laboratory research at White Mountain
Research Station during the summer and laboratory
biochemical research at Sonoma State University and Santa
Clara University during the winter.
I am looking for a Masters student to participate in this
research starting in Summer 1999. Support for this research
from the National Science Foundation is pending from a
proposal submitted in January 1999. Additional support for
graduate student research may be available from White
Mountain Research Station and Sonoma State University.
Sonoma State University has an active Master's program that
emphasizes graduate student research in a number of areas in
organismal and molecular biology.
During the summer of 1999, we will
conduct the following field studies:
- We will collect adult beetles of
known body temperatures along altitudinal gradients in
new drainages in the Sierra Nevada Mountains.
- We will conduct a larval transplant
experiment to determine whether beetle larvae survive and
perform best in their natal drainage.
During the follwoing year, we will
- Isolate and purify PGI from
- Measure stress protein levels and
physiological parameters for beetles from the transplant
- Use allozyme electrophoresis to
quantify genetic variation among new populations of leaf
Biology | Sonoma State
January 23, 1999 NER